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KMID : 0371619990150010069
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Journal of Wonkwang Medical Science
1999 Volume.15 No. 1 p.69 ~ p.76
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The Study of Enzyme Activity in Liver Fibrosis Induced Rat That was Treated with Cordyceps Sinensis(Dongchunghacho)
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Kim Sang-Deok
Lee Geon-Mok
Kim Ki-Young
Lee Ki-Nam
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Abstract
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Purpose: We study on the effects of Cordyceps sinensis (Dongchunghacho: DCHC) about activity of hepatic enzyme, which is presumed to be effective on the liver disease at Oriental Medicine.
Methods: In the study, the antifibrotic effects of DCHC was investigated by oral applicalion on liver fibrosis induced in rats. Female Sprague Dawly rats were divided in 3 groups (control, liver fibrosis induced group, liver fibrosis induced and DCHC oral application group). For liver fibrosis induction, rats were i. p. injected in 11 weeks (0.125㎖/rat/day). Rats were treated for 10 weeks with DCHC extracts(0.16g/㎏/day). After application of DCHC, all group rats were sacrified. and collected blood and liver tissue. After centrifugation of blood, sera was used for the measurement of clinical biochemistry (ALT, AST, ALP, total bilirubin). The parameter of collagen deposition in liver tissue (hydroxyproline: HYP) was measured by a colorimetry.
Results: In the result, jaundice was not observed in all groups, but was showed hepatomegaly in CCl4 treated group and CCl4/DCHC applicated group. The ratio of body liver weight of CCl4 treated rats was significantly higher than in DCHC treated rats (5.2±0.4%, 4.0±0.3%, p<0.01), but the weights of spleen and kidney in all group investigated were not changed significantly. The content of HYP was significantly lower in CCl4 with DCHC group than in CCl4 group (642.0±54.0, 688.0±30.5 ㎍/1g, p<0.05). In DCHC treated group, the values of AST, ALP, BUN, γ-GTP were very low compared with only CCl4 intoxificated group(p<0.01). This result represents that CCl4 injection induced liver fibrosis in 11 weeks. DCHC inhibited significantly the accumulation of collagen in the extracellular space and the activities of AST, ALT, ALP, γ-GTP in serum.
Conclusion: It conclude that DCHC extracts is proven to protect against liver fibrosis in rat and to stimulate liver function.
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KEYWORD
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Liver Fibrosis, Cordyceps sinensis(Dongchunghacho), Liver Enzyme
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